Development of an oncogenic dedifferentiation SOX signature with prognostic significance in hepatocellular carcinoma.
Gradual loss of terminal differentiation markers and benefits of stem cell-like properties is the main hall mark of the development of malignant cancer. Pluripotent stem cells SOX family transcription factors play important role in regulating tumor plasticity and specifications descent.
The purpose of this study to establish the new SOX signature to monitor the extent of the dedifferentiation of the tumor and predict the prognostic significance in hepatocellular carcinoma (HCC) .the RNA-seq data from The Cancer Genome Atlas (TCGA) project LIHC chronologically divided into training (n = 188) and testing cohort (n = 189).
Liri-JP project of the International Cancer Genome Consortium (ICGC) data portal is used as an independent validation cohort (n = 232). Kaplan-Meier and Cox multivariable analysis was used to test the clinical significance and prognostic value of the signature genes.The SOX gene family members found aberrantly expressed in HCC patients clinically. A five SOX gene signature with established prognostic value in the training group. The SOX gene signatures are found closely associated with tumor grade and stage of the tumor.
markers dedifferentiation of liver cancer (AFP, CD133, EpCAM, and KRT19) found increased while a marker of hepatocyte terminal differentiation (ALB, G6PC, CYP3A4, and HNF4A) decreasing from HCC patients with a score of signatures SOX lower for patients with a high score SOX signature. Kaplan-Meier survival analysis further shows that the newly founded signature SOX excited to predict the overall survival of patients in both the training, testing and independent validation of oncogenic dedifferentiation cohort.An SOX signature gift great potential in predicting the prognostic significance in HCC, and may provide a novel biomarker for further precision in clinical oncology.
Development of an oncogenic dedifferentiation SOX signature with prognostic significance in hepatocellular carcinoma.
Update on legacy and Gastrointestinal polyposis colon cancer.
It is estimated that 5-10% of colorectal cancers arise due to known genetic syndromes. Individuals with this cancer syndrome ekstrakolon cancer risk. Polyposis and hereditary nonpolyposis syndrome is generally recognized. The entry of next generation sequencing technology, particularly multiple-gene panel test, in routine laboratory practice have been made to identify the cause of the disease is significantly easier.To summarizes current knowledge of the causes, clinical manifestations, diagnostic criteria, and recommendations for the presymptomatic screening of individuals at risk of gastrointestinal polyposis and hereditary colorectal cancer syndromes. We dicuss currently defined syndrome is detected by multiple-gene panel generation sequencing;
These include constitutional deficiencies repair mismatches (mutations of genes MLH1, MSH2, MSH6, PMS2 biallelic), gastric adenocarcinoma and polyps proximal stomach (APC gene), polyposis NTHL1 related, polymerase proofreading associated polyps (POLD1, gene POLE), juvenile polyposis (SMAD4 , gene BMPR1A), and serrated polyposis syndrome.
Another aim is to summarize the latest knowledge about well-known syndromes, including heredity nonpolyposis bowel cancer (Lynch syndrome), familial adenomatous polyposis, MUTYH associated polyposis, and Peutz-Jeghers and Cowden / PTEN hamartoma tumor syndromes.Awareness of descent polyposis / bowel cancer syndromes allow early diagnosis and prevention of cancer in affected individuals and their families.
Description: C-Jun-amino-terminal kinase-interacting protein 3 encoded by MAPK8IP3 shares similarity with the product of Drosophila syd gene, required for the functional interaction of kinesin I with axonal cargo. Studies of the similar gene in mouse suggested that this protein may interact with, and regulate the activity of numerous protein kinases of the JNK signaling pathway, and thus function as a scaffold protein in neuronal cells. The C. elegans counterpart of this gene is found to regulate synaptic vesicle transport possibly by integrating JNK signaling and kinesin-1 transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
Description: C-Jun-amino-terminal kinase-interacting protein 3 encoded by MAPK8IP3 shares similarity with the product of Drosophila syd gene, required for the functional interaction of kinesin I with axonal cargo. Studies of the similar gene in mouse suggested that this protein may interact with, and regulate the activity of numerous protein kinases of the JNK signaling pathway, and thus function as a scaffold protein in neuronal cells. The C. elegans counterpart of this gene is found to regulate synaptic vesicle transport possibly by integrating JNK signaling and kinesin-1 transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
Description: C-Jun-amino-terminal kinase-interacting protein 3 encoded by MAPK8IP3 shares similarity with the product of Drosophila syd gene, required for the functional interaction of kinesin I with axonal cargo. Studies of the similar gene in mouse suggested that this protein may interact with, and regulate the activity of numerous protein kinases of the JNK signaling pathway, and thus function as a scaffold protein in neuronal cells. The C. elegans counterpart of this gene is found to regulate synaptic vesicle transport possibly by integrating JNK signaling and kinesin-1 transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
Description: A polyclonal antibody for detection of JIP-1 from Human, Mouse, Rat. This JIP-1 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human JIP-1 around the non-phosphorylation site of T103
Description: A polyclonal antibody for detection of JIP-1 from Human, Mouse, Rat. This JIP-1 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human JIP-1 around the non-phosphorylation site of T103
Description: A polyclonal antibody for detection of JIP-1 from Human, Mouse, Rat. This JIP-1 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human JIP-1 around the non-phosphorylation site of T103
Description: A polyclonal antibody for detection of JIP-2 from Human, Mouse, Rat. This JIP-2 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human JIP-2 at AA rangle: 550-630
Description: A polyclonal antibody for detection of JIP-2 from Human, Mouse, Rat. This JIP-2 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human JIP-2 at AA rangle: 550-630
Description: A polyclonal antibody for detection of JIP-2 from Human, Mouse, Rat. This JIP-2 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human JIP-2 at AA rangle: 550-630
Description: The mitogen-activated protein kinase 8 interacting protein 2 encoded by MAPK8IP2 is closely related to MAPK8IP1/IB1/JIP-1, a scaffold protein that is involved in the c-Jun amino-terminal kinase signaling pathway. This protein is expressed in brain and pancreatic cells. It has been shown to interact with, and regulate the activity of MAPK8/JNK1, and MAP2K7/MKK7 kinases. This protein thus is thought to function as a regulator of signal transduction by protein kinase cascade in brain and pancreatic beta-cells.
Description: The mitogen-activated protein kinase 8 interacting protein 2 encoded by MAPK8IP2 is closely related to MAPK8IP1/IB1/JIP-1, a scaffold protein that is involved in the c-Jun amino-terminal kinase signaling pathway. This protein is expressed in brain and pancreatic cells. It has been shown to interact with, and regulate the activity of MAPK8/JNK1, and MAP2K7/MKK7 kinases. This protein thus is thought to function as a regulator of signal transduction by protein kinase cascade in brain and pancreatic beta-cells.
Description: The mitogen-activated protein kinase 8 interacting protein 2 encoded by MAPK8IP2 is closely related to MAPK8IP1/IB1/JIP-1, a scaffold protein that is involved in the c-Jun amino-terminal kinase signaling pathway. This protein is expressed in brain and pancreatic cells. It has been shown to interact with, and regulate the activity of MAPK8/JNK1, and MAP2K7/MKK7 kinases. This protein thus is thought to function as a regulator of signal transduction by protein kinase cascade in brain and pancreatic beta-cells.
Description: MAPK8IP1 encodes a regulator of the pancreatic beta-cell function. C-Jun-amino-terminal kinase-interacting protein 1 is highly similar to JIP-1, a mouse protein known to be a regulator of c-Jun amino-terminal kinase (Mapk8). C-Jun-amino-terminal kinase-interacting protein 1 has been shown to prevent MAPK8 mediated activation of transcription factors, and to decrease IL-1 beta and MAP kinase kinase 1 (MEKK1) induced apoptosis in pancreatic beta cells. This protein also functions as a DNA-binding transactivator of the glucose transporter GLUT2. RE1-silencing transcription factor (REST) is reported to repress the expression of this gene in insulin-secreting beta cells. This gene is found to be mutated in a type 2 diabetes family, and thus is thought to be a susceptibility gene for type 2 diabetes.
Description: MAPK8IP1 encodes a regulator of the pancreatic beta-cell function. C-Jun-amino-terminal kinase-interacting protein 1 is highly similar to JIP-1, a mouse protein known to be a regulator of c-Jun amino-terminal kinase (Mapk8). C-Jun-amino-terminal kinase-interacting protein 1 has been shown to prevent MAPK8 mediated activation of transcription factors, and to decrease IL-1 beta and MAP kinase kinase 1 (MEKK1) induced apoptosis in pancreatic beta cells. This protein also functions as a DNA-binding transactivator of the glucose transporter GLUT2. RE1-silencing transcription factor (REST) is reported to repress the expression of this gene in insulin-secreting beta cells. This gene is found to be mutated in a type 2 diabetes family, and thus is thought to be a susceptibility gene for type 2 diabetes.
Description: MAPK8IP1 encodes a regulator of the pancreatic beta-cell function. C-Jun-amino-terminal kinase-interacting protein 1 is highly similar to JIP-1, a mouse protein known to be a regulator of c-Jun amino-terminal kinase (Mapk8). C-Jun-amino-terminal kinase-interacting protein 1 has been shown to prevent MAPK8 mediated activation of transcription factors, and to decrease IL-1 beta and MAP kinase kinase 1 (MEKK1) induced apoptosis in pancreatic beta cells. This protein also functions as a DNA-binding transactivator of the glucose transporter GLUT2. RE1-silencing transcription factor (REST) is reported to repress the expression of this gene in insulin-secreting beta cells. This gene is found to be mutated in a type 2 diabetes family, and thus is thought to be a susceptibility gene for type 2 diabetes.
Description: A polyclonal antibody for detection of JIP-1 phospho Thr103) from Human, Mouse, Rat. This JIP-1 phospho Thr103) antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human JIP-1 around the phosphorylation site of T103
Description: A polyclonal antibody for detection of JIP-1 phospho Thr103) from Human, Mouse, Rat. This JIP-1 phospho Thr103) antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human JIP-1 around the phosphorylation site of T103
Description: A polyclonal antibody for detection of JIP-1 phospho Thr103) from Human, Mouse, Rat. This JIP-1 phospho Thr103) antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human JIP-1 around the phosphorylation site of T103
Description: This gene encodes a regulator of the pancreatic beta-cell function. It is highly similar to JIP-1, a mouse protein known to be a regulator of c-Jun amino-terminal kinase (Mapk8). This protein has been shown to prevent MAPK8 mediated activation of transcription factors, and to decrease IL-1 beta and MAP kinase kinase 1 (MEKK1) induced apoptosis in pancreatic beta cells. This protein also functions as a DNA-binding transactivator of the glucose transporter GLUT2. RE1-silencing transcription factor (REST) is reported to repress the expression of this gene in insulin-secreting beta cells. This gene is found to be mutated in a type 2 diabetes family, and thus is thought to be a susceptibility gene for type 2 diabetes.
Description: This gene encodes a regulator of the pancreatic beta-cell function. It is highly similar to JIP-1, a mouse protein known to be a regulator of c-Jun amino-terminal kinase (Mapk8). This protein has been shown to prevent MAPK8 mediated activation of transcription factors, and to decrease IL-1 beta and MAP kinase kinase 1 (MEKK1) induced apoptosis in pancreatic beta cells. This protein also functions as a DNA-binding transactivator of the glucose transporter GLUT2. RE1-silencing transcription factor (REST) is reported to repress the expression of this gene in insulin-secreting beta cells. This gene is found to be mutated in a type 2 diabetes family, and thus is thought to be a susceptibility gene for type 2 diabetes.
Description: This gene encodes a regulator of the pancreatic beta-cell function. It is highly similar to JIP-1, a mouse protein known to be a regulator of c-Jun amino-terminal kinase (Mapk8). This protein has been shown to prevent MAPK8 mediated activation of transcription factors, and to decrease IL-1 beta and MAP kinase kinase 1 (MEKK1) induced apoptosis in pancreatic beta cells. This protein also functions as a DNA-binding transactivator of the glucose transporter GLUT2. RE1-silencing transcription factor (REST) is reported to repress the expression of this gene in insulin-secreting beta cells. This gene is found to be mutated in a type 2 diabetes family, and thus is thought to be a susceptibility gene for type 2 diabetes.
Description: A Rabbit Polyclonal antibody against JIP-1 (phospho Thr103) from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA
Description: A Rabbit Polyclonal antibody against JIP-1 (phospho Thr103) from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA
Description: A polyclonal antibody for detection of JIP4 from Human, Mouse. This JIP4 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human JIP4 protein at amino acid sequence of 590-670
Description: A polyclonal antibody for detection of JIP4 from Human, Mouse. This JIP4 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human JIP4 protein at amino acid sequence of 590-670
Description: A polyclonal antibody for detection of JIP4 from Human, Mouse. This JIP4 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human JIP4 protein at amino acid sequence of 590-670
Description: This gene encodes a member of the cancer testis antigen gene family. The encoded protein functions as a scaffold protein that structurally organizes mitogen-activated protein kinases and mediates c-Jun-terminal kinase signaling. This protein also binds to kinesin-1 and may be involved in microtubule-based membrane transport. This protein may play a role in tumor growth and development. Alternate splicing results in multiple transcript variants.
Description: This gene encodes a member of the cancer testis antigen gene family. The encoded protein functions as a scaffold protein that structurally organizes mitogen-activated protein kinases and mediates c-Jun-terminal kinase signaling. This protein also binds to kinesin-1 and may be involved in microtubule-based membrane transport. This protein may play a role in tumor growth and development. Alternate splicing results in multiple transcript variants.
Description: This gene encodes a member of the cancer testis antigen gene family. The encoded protein functions as a scaffold protein that structurally organizes mitogen-activated protein kinases and mediates c-Jun-terminal kinase signaling. This protein also binds to kinesin-1 and may be involved in microtubule-based membrane transport. This protein may play a role in tumor growth and development. Alternate splicing results in multiple transcript variants.
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human JIP3 . This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody for detection of 14-3-3 ? from Human, Mouse, Rat. This 14-3-3 ? antibody is for WB , IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human 14-3-3 ? at AA range: 180-260
Description: A polyclonal antibody for detection of 14-3-3 ? from Human, Mouse, Rat. This 14-3-3 ? antibody is for WB , IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human 14-3-3 ? at AA range: 180-260
Description: A polyclonal antibody for detection of 14-3-3 ? from Human, Mouse, Rat. This 14-3-3 ? antibody is for WB , IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human 14-3-3 ? at AA range: 180-260
Description: A polyclonal antibody for detection of 14-3-3 ? from Mouse, Rat. This 14-3-3 ? antibody is for WB, IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein
Description: A polyclonal antibody for detection of 14-3-3 ? from Mouse, Rat. This 14-3-3 ? antibody is for WB, IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein
Description: A polyclonal antibody for detection of 14-3-3 ? from Mouse, Rat. This 14-3-3 ? antibody is for WB, IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein
Description: A polyclonal antibody for detection of 14-3-3 ? from Human, Mouse. This 14-3-3 ? antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human 14-3-3 ? at AA rangle: 10-90
Description: A polyclonal antibody for detection of 14-3-3 ? from Human, Mouse. This 14-3-3 ? antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human 14-3-3 ? at AA rangle: 10-90
Description: A polyclonal antibody for detection of 14-3-3 ? from Human, Mouse. This 14-3-3 ? antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human 14-3-3 ? at AA rangle: 10-90
Description: A polyclonal antibody for detection of 14-3-3 ? from Human, Mouse, Rat. This 14-3-3 ? antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human 14-3-3 ? at AA range: 30-110
Description: A polyclonal antibody for detection of 14-3-3 ? from Human, Mouse, Rat. This 14-3-3 ? antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human 14-3-3 ? at AA range: 30-110
Description: A polyclonal antibody for detection of 14-3-3 ? from Human, Mouse, Rat. This 14-3-3 ? antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human 14-3-3 ? at AA range: 30-110
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human JIP3 / Syd2 / JSAP1 (N-Term). This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human 14-3-3 ε . This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human 14-3-3 θ . This antibody is tested and proven to work in the following applications:
genetic counseling, presymptomatic testing of at-risk individuals, and efficient screening may be helpful to affected families. Thanks to Lenka Foretová, M.D., Ph.D., (Masaryk Memorial Cancer Institute, Brno) for a critical review of the manuscript and valuable advice. The author states he has no potential conflict of interest concerning medicines, products, or services used in this study. Editorial Board that the text met ICMJE recommendation for biomedical papers.
